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HelpCatalog ID FPHIV Phenosense HIV Drug Resistance Replication Capacity
Reporting Name
Phenosense HIVOrdering Guidance
This procedure should be used for patients with documented HIV-1 infection and viral loads ≥500 copies/mL.
Specimen Required
Specimen Type: Plasma
Container/Tube: Lavender-top (EDTA) or plasma preparation tube (PPT)
Specimen Volumes: 3 mL
Collection Instructions: Draw blood into two 5-mL PPT (pearl top) or EDTA (lavender top) tubes. Centrifuge specimen within 6 hours of collection. Transfer plasma to one or more polypropylene screw-capped tube(s) and freeze. Freeze immediately. Send 3 mL plasma in a screw-cap vial frozen.
To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.
RECOMMENDED:
1. Patient's most recent viral load.
2. Viral load collection date.
NOTE:
1. Intended for use only for patients with viral loads greater than or equal to 500 copies/mL. For best results, viral loads should be confirmed within two weeks prior to submission for testing at Monogram.
2. Patient samples submitted <30 days apart are considered duplicate and will be canceled.
Collect 2 full Purple top tubes.
Day(s) Performed
Sunday through Saturday
Reference Values
A final report will be attached in MayoAccess.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 14 days |
Performing Laboratory
Monogram Biosciences, IncSpecimen Type
Plasma EDTACPT Code Information
87903
87904 x12
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FPHIV | Phenosense HIV | 49665-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| Z1042 | Phenosense HIV | Unable to Verify |
Reject Due To
| Other | PPT not centrifuged; insufficient volume; heparinized plasma; non frozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap- cap" tube |
Method Name
Polymerase chain reaction (PCR) amplification and viral culture
Report Available
18 to 26 daysUseful For
Determines viral phenotype resistance to three classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), non-nucleoside reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI). Viral replication capacity is also included.