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Catalog ID EBVIN EBV inSIGHT T Cell Immunity

Important Note

Can only collect specimens between 8am-3pm, Monday - Friday, and not on or day before a FedEx holiday

Specimen must ship same day and arrive at Eurofins Viracor within 32 hours of specimen collection

Additional Codes

CPOE: Ref Spec Misc; specigy EBV Insight

Specimen

Sodium Heparin Green top tube, collect 10.0 mL blood, tube must be at least 3/4 full!

Whole blood, 10.0 mL (4.0 mL for peds only), room temperature (must arrive at Eurofins Viracor <32 hours from collection!!!)

Rejection Criteria

Plasma, serum, other anticoagulants, refrigerated specimen, frozen specimen, tube not at least 3/4 full, specimen >32 hours when received by Eurofins Viracor

Interpretative Information

Procedure

Whole blood is stimulated with the positive control SEB, EBV specific antigens (EBV lysate, BZLF1 and EBNA 3) or left unstimulated as a negative control and incubated to 37ºC. During the incubation Brefeldin A is added causing the cytokines (Interferon – gamma (IFN-γ) Tumor Necrosis Factor – alpha (TNF-α) and/or Interleukin 2 (IL-2)) to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for cellular markers (CD3, CD4, CD8, CD69), the cytokines above and analyzed by flow cytometry. Test will measure the % Interferon-gamma and polyfunctional index responses to the EBV lytic antigen BZLF1, EBV latent antigens EBNA 3A/3C and an EBV infected cell lysate.

Performing Lab

Eurofins Viracor - test code 33217

Turn-Around-Time

4-5 days

Reference Values

See report