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Catalog ID DAT Panel Direct Coombs Panel

CPOE Test Name

Direct Antihuman Globulin Panel, DAT Panel

Synonyms

Broad Spectrum Direct Coombs, Direct Antihuman Globulin Test

Specimen Requirements

Samples require Name, MRN, date and phlebotomist initials/Cerner ID. 1 lavender top (EDTA) or pink top (EDTA) tube.

Storage and Transport

Send to Blood Bank

Causes for Rejection

Improper labeling; wrong tube; gross hemolysis; insufficient volume

Interpretation

A positive DAT indicates that antibody and/or complement is coating the red blood cells unless a false positive result has occurred. Requires elution and panel testing to determine the reason.

Turn Around Time

Panel 30 minutes

Methodology

Uses Broad Spectrum, IgG, and C3d antihuman globulin serums in test procedure with patient’s washed RBCs.

CPT / HCPCS Code

86880

Reference Ranges

Critical Value is a Positive DAT of 1+ or greater

Performing Lab

Blood Bank BI-1230 721-2731