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Catalog ID CHF8 Chromogenic Factor VIII Activity Assay, Plasma


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, a coagulation consultation is recommended.



Necessary Information


 



Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, at -40° C or below.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Secondary ID

610419

Useful For

Monitoring coagulation factor replacement therapy of selected extended half-life coagulation factor replacements

 

Aiding in the diagnosis of hemophilia A using a 2-stage assay, especially when the 1-stage assay result was normal

Testing Algorithm

This assay is indicated in situations where there is a clinical suspicion of hemophilia A diagnosis, but the 1-stage factor VIII (FVIII) assay result is normal. However, recent guidelines recommend this assay be performed in addition to the 1-stage assay in the initial workup of hemophilia A.

 

Testing for autoantibodies to FVIII in the presence of a low FVIII activity may be clinically indicated. For adding on FVIII inhibitor, call 800-533-1710 within 7 days to assess if adequate plasma sample is available.

 

For more information see Hemophilia Testing Algorithm.

Method Name

Chromogenic

Reporting Name

Chromogenic FVIII, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
IV heparin contamination Reject

Reference Values

55.0-200.0%

 

Chromogenic factor VIII activity generally correlates with the one-stage FVIII activity. In full term/premature neonates, infants, children, and adolescents the one-stage FVIII activity* is similar to adults. However, no similar data for chromogenic FVIII activity are available.(Appel IM, Grimminck B, Geerts J, Stigter R, Cnossen MH, Beishuizen A. Age dependency of coagulation parameters during childhood and puberty. J Thromb Haemost. 2012;10(11):2254-63)

 

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

85130

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHF8 Chromogenic FVIII, P 49865-9

 

Result ID Test Result Name Result LOINC Value
CHF8 Chromogenic FVIII, P 49865-9

Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.