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Catalog ID BCLL IGH Somatic Hypermutation Analysis, B-Cell Chronic Lymphocytic Leukemia (B-CLL), Varies

Useful For

Providing prognostic information in patients with newly diagnosed B-cell chronic lymphocytic leukemia

 

This test is not intended for use in providing prognostic information for patient with other B-cell neoplasms or hematopoietic tumors.

Reporting Name

IGH Somatic Hypermutation in B-CLL

Specimen Type

Varies


Shipping Instructions


1. Both refrigerated and ambient specimens must arrive within 7 days of collection.

2. Collect and package specimen as close to shipping time as possible.



Necessary Information


1. Molecular Hematopathology Patient Information: B-Cell Chronic Lymphocytic Leukemia (CLL) for IGVH and/or TP53 Somatic Mutation Testing (T711) is required, see Special Instructions. Testing may proceed without the patient information, however, it aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.

2. If form is not provided, include the following information with the test request: specimen source, pertinent clinical history (ie, CBC results and relevant clinical notes), and clinical or morphologic suspicion.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Peripheral blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

Specimen Stability: Refrigerated/ Ambient

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

Specimen Stability: Refrigerated/ Ambient

 

 

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL screw-top tube

Specimen Volume: Entire specimen

Collection Instructions:

1. Label specimen as extracted DNA and indicate specimen source (blood or bone marrow).

2. The required volume of DNA is 50 mcL at a concentration of 20 ng/mcL

3. Include volume and concentration on tube.

Specimen Stability: Frozen (preferred)/Refrigerated


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies 7 days

Reject Due To

Gross hemolysis Reject
Other Moderately to severely clotted

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday, Wednesday, Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81263-IGH (immunoglobulin heavy chain locus) (eg, leukemia and lymphoma, B-cell), variable region somatic mutation analysis

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BCLL IGH Somatic Hypermutation in B-CLL In Process

 

Result ID Test Result Name Result LOINC Value
39465 BCLL Result No LOINC Needed
MP005 Specimen Type 31208-2
19674 Final Diagnosis 50398-7

Method Name

Polymerase Chain Reaction (PCR) and Next-Generation Sequencing

Secondary ID

89008

Specimen Minimum Volume

Blood: 1 mL
Bone Marrow: 1 mL
Extracted DNA: see Specimen Required

Forms

1. Molecular Hematopathology Patient Information: B-Cell Chronic Lymphocytic Leukemia (CLL) for IGVH and/or TP53 Somatic Mutation Testing (T711) is required, see Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.