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Catalog ID AbID Antibody Identification

Important Note

Reflex test that may be necessary prior to transfusion to identify antibody or antibodies when the antibody screen test is positive. May be done on eluates from red cells having a positive DAT test. Necessary to work up obstetric patients for antenatal diagnosis of HDN. To work up hemolytic anemia when the DAT and or indirect Coombs’ test is positive.

CPOE Test Name

Ordered by Blood Bank. 

Synonyms

Antibody panel, antigen testing, red cell antibody identification, elution, DAT and etc.

Specimen Requirements

Samples require Name, MRN, date and phlebotomist initials/Cerner ID. Pink top (EDTA) tube. (Both a lavender and red stoppered tube may be requested if more tests are required for identification purposes).

Storage and Transport

Send to Blood Bank

Causes for Rejection

Improper labeling; wrong tube; gross hemolysis; insufficient volume

Interpretation

Difficulty in crossmatching patients sensitized to red cell antigens may result in delay of blood availability.? Identified clinically significant antibodies known to cause HDN will be titered if identified in obstetric patients.

Turn Around Time

Simple: 60 minutes
Complex: 2-3 hours
Referral: > 24 hours

Methodology

Immediate spin, 37C phase using LISS, then indirect Coombs’ phase.? May require adsorption and elution procedures, selected panels, and other mediums for identification purposes.

CPT / HCPCS Code

86885, 86880, 86900, 86870, 86886, 86850, 86860

Reference Ranges

Not applicable

Performing Lab

Blood Bank BI 1230 721-2731